Skills

• Molecular biology and biotechnology

Skillful in preparation and isolation of nucleic acids, Gel electrophoresis, Polymerase chain reaction, real time polymerase chain reaction, restriction digestion and analysis, Gene Cloning and protein expression highly skilled in SDS-PAGE analysis, western blotting, protein purification specifically via affinity chromatography using Ni-NTA matrix.

• Bioinformatics

Skillful in working with Snap Gene, AllelelID7, Oligo Analyzer, Oligo7, Gene runner and online programs for designing primers such as primer3, NCBI Primer Blast. Adept in using data bases including Uniports, NCBI etc.

• Cell culture

Animal cell culture and cytotoxicity assays and flow cytometer

Publications

1. S. Abdolmohammadi-vahid, A. Naser, F. Chakari-Khiavi, Dr Maryam Darayee, K. Berjis, L. Aghebati-Maleki, N. Najdi, J. Ahmadian-Heris, M. Yousefi. Vaginal probiotics and vitamin D, as immunomodulatory approaches in improving the implantation and live birth in repeated implantation failure (RIF) women with imbalanced. immune system. 40th Hybrid Annual Meeting of the ESHRE, Amsterdam – The Netherlands, 7-10 July 2024
2. Farnaz Sani, Mahsa Sani, Zahra Moayedfard, Dr Maryam Darayee, Lobat Tayebi, Negar Azarpira. (May 2023) Potential advantages of genetically modified mesenchymal stem cells in the treatment of acute and chronic liver diseases. Stem Cell Research & Therapy, volume 14, issue 1, page 138.
3. Dr Maryam Darayee, Bita Geramizadeh, Seyed Mohammad Bagher Tabei, Alireza Rezvani, Seeede Soleimanian, Amir Rahimi. (Jun 2022) Suggesting Tissue-Specific MSMB Gene Promoter as a Novel Approach for Prostate Targeted Gene Therapy. Asian Pacific Journal of Cancer Prevention: APJCP,volume 23, issue 6, page 1993.
4. Hossein Jafari Khamirani, Ali Saber Sichani, Seyed Mehdi Panahandeh, Sina Zoghi, Seyed Sajjad Tabei, Dr Maryam Darayee, Mahdieh Talebzadeh, Mehdi Dianatpour, Seyed Alireza Dastgheib, Seyed Mohammad Bagher Tabei. (Jun 2022). EXOSC9 mutation causes pontocerebellar hypoplasia type 1D (PCH1D): Refining the phenotype and literature review. Gene Reports, volume 27 page 101568.
5. Mehran Piran, Dr Maryam Darayee, Mehrdad Piran, Neda Sepahi, Amir Rahimi. (Jan 2020) Gene Expression Signature and Molecular Mechanism of Redox Homeostasis in Colorectal Cancer. BioRxiv, page 28.920553
6. Darayee M, Shanehbandi D, Darayee M, Sadeghi Shabestari M, Ghojazadeh M, Kazemi T, Babaloo Z*. (Jun 2015) Evaluation of FOXP3 gene polymorphisms in patients with allergic disease compared with controls. Jokul 65(6).

Book

• Co-author of book “comprehensive immunology for non-immunology student” Khalili publication, Tehran, Iran, 2014.

Fields of interest

Reproductive Immunology, Immunotherapy for recurrent pregnancy loss and repeated implantation failure, Gene therapy.